Blood can also be taken from an EDTA sample. The page below is a sample from the LabCE course Red Cell Disorders: Peripheral Blood Clues to Nonneoplastic Conditions (retired 12/30/2011). Peripheral blood smear List laboratory data (WBC, Hemoglobin, Hematocrit, MCV, Platelet count, RDW, other) Give microscopic description (number, size, shape, other abnormalities) of red blood cells, white blood cells and platelets Simply entering findings such as cell count or even morphologic features is not sufficient to generate a report. Peripheral blood smear examination is an invaluable investigation for the diagnosis of various clinical diseases, it includes cellular description of various blood cells seen on slide. 0000277006 00000 n This synoptic reporting system can be accessed at the following Web sites: http://HemePathReview.com/ and http://www.uth.tmc.edu/pathology/faculty/pages/nguyen-nghia/decision.html. For full access to this pdf, sign in to an existing account, or purchase an annual subscription. Place a small drop of blood, or one side about 1-2 cm from one end. A physician-initiated request for a blood smear is usually a response to perceived clinical features or to an abnormality shown in a previous complete blood count. 0000224310 00000 n 0000279387 00000 n It cannot be overemphasized that clinical judgment is the most important element in finalizing the report. These slides were analysed with Shonit™. trailer We will continue to have residents voluntarily use the system while rotating on our hematopathology service. of collection) Preparation of Blood Film: The slide should be clean. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online. Blood Work is an educational instrument to physicians and hematology students. 0000009179 00000 n Peripheral Blood Smear. Unlike other systems, there is only 1 main window and the user is not required to scroll through multiple menus, windows, and drop-down lists. Review of peripheral blood smear is also very important, and sometimes correlating abnormal findings of bone marrow biopsy with abnormalities observed during review of peripheral blood smear may be very helpful for obtaining a proper diagnosis. The figure must clearly demonstrate the feature(s) being described and the discussion should emphasize this teaching point(s). A well-stained smear is also important. 0000281023 00000 n AL Method Microscopy . 0000275246 00000 n 0000007577 00000 n MO ×100 objective) should be stated within the text. Proper preparation of the blood smear is critical for correct interpretation and obtaining accurate results. In this way, residents learn to see the peripheral blood smear report not only as a list of findings but also as an important tool for patient evaluation and treatment. 0000004563 00000 n 0000221280 00000 n 0000219111 00000 n Exclusion of important information in the drafts was also decreased, as observed by the attending hematopathologists signing out the final reports. E.D.T.A. Google Scholar | Crossref | Medline. The ASCP is accredited by the Accreditation Council for Continuing Medical Education to provide continuing medical education for physicians. De If you are using venous blood, blood smears should be prepared as soon as possible after collection (delay can result in changes in parasite morphology and staining characteristics). 0000013712 00000 n 0000210554 00000 n Also, the interface is very user friendly and intuitive enough that users can become adept at its use with only a few sessions. 0000245295 00000 n Any inclusion bodies (eg, Howell-Jolly bodies or malarial parasites) will also be noted. 0000278646 00000 n 0000218441 00000 n Examining multiple smears of the same blood sample is also helpful because alterations that are inconstant from smear to smear are likely to be artifactual. Blood smears are needed for microscopic examination of the blood. 0000024991 00000 n Nguyen Blood smear is prepared, stained with Leishman’s stain and cells are identified under oil immersion lens. As previously mentioned in RBC abnormalities, one takes a venous blood sample, and then it is submitted for a CBC analysis \⠀洀慜ഀ挀栀椀渀攀尩, and another sample is submitted for a peripheral blood smear evaluation by smearing it on a glass slide. Internal reflections or contamination in the sample and/or in the optics can cause refractiles. (3) This reporting system is accessed on the Internet. The smear shows numerous target cells and a spur cell (top right). Alternative Names. 0000005596 00000 n 0000007998 00000 n Oxford University Press is a department of the University of Oxford. 0000277536 00000 n 0000281423 00000 n CPT, Current Procedural Terminology code; DAT, direct antiglobulin test; LDH, lactate dehydrogenase; PMNs, polymorphonuclear leukocytes. In this way, we can gather the perspectives and advice of a wide array of users, which will allow us to improve the system accordingly. Selection of a spreader: Take one slide a spreader which has smooth edge. 0000297784 00000 n et al. Checklists, such as those provided by the College of American Pathologists, are frequently used to generate surgical pathology reports.2 Web-based synoptic reporting systems that incorporate such checklists have been shown to improve efficiency, reduce turnaround time, and decrease reporting errors.3 Synoptic systems for hematologic neoplasms and bone marrow reporting have also been described with similar results.4,5 To date, such a system has not been described for the reporting of peripheral blood smear findings. 0000009934 00000 n Crenated Erythrocytes Crenated erythrocytes (see Figure 26-36), artifacts especially common in feline blood samples, have a thorn-apple shape with many short, uniformly spaced, blunt or pointed spicules that protrude from the cell membrane. 0000277112 00000 n 0000146506 00000 n xref Users can access this system on the Internet and select relevant attributes from drop-down lists to obtain a short list of report templates with findings that match those of the case under consideration. 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